The advantages of the method for extracting hyaluronic acid comprise that the operation technology is simple, raw materials are cheap and easy to obtain, the waste utilization and comprehensive exploitation effects are given play, and a road is opened up for exploiting high-added-value products. The method comprises the following steps: peeling and getting tuna eye vitreous body from wastes generated after tuna is sliced, performing acetone degreasing, drying and crushing adding distilled water into tuna eye dry powder for extraction, and centrifuging to obtain a supernatant employing an enzyme membrane reactor for processing performing ethanol precipitation dissolving the precipitate, performing complexing precipitation by using a complexing agent, dissociating the precipitate by using a salt, centrifuging, performing ethanol precipitation on the dissociation solution, and finally performing dewatering and drying on the precipitate, so as to obtain the hyaluronic acid product. The invention provides an extraction method for hyaluronic acid in tuna eye. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by H&P FOODS Ltd, Zhejiang University of Technology ZJUT filed Critical H&P FOODS Ltd Priority to CN201510510730.0A priority Critical patent/CN105131150B/en Publication of CN105131150A publication Critical patent/CN105131150A/en Application granted granted Critical Publication of CN105131150B publication Critical patent/CN105131150B/en Status Expired - Fee Related legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee H&P FOODS Ltd Zhejiang University of Technology ZJUT Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 应国清 王婧茜 陈建澍 易喻 梅建凤 张彦璐 龚海平 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN201510510730.0A Other languages Chinese ( zh) Google Patents Extraction method for hyaluronic acid in tuna eyeĭownload PDF Info Publication number CN105131150A CN105131150A CN201510510730.0A CN201510510730A CN105131150A CN 105131150 A CN105131150 A CN 105131150A CN 201510510730 A CN201510510730 A CN 201510510730A CN 105131150 A CN105131150 A CN 105131150A Authority CN China Prior art keywords tuna hyaluronic extracting method hyaluronic acid aqueous solution Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN105131150A - Extraction method for hyaluronic acid in tuna eye CN105131150A - Extraction method for hyaluronic acid in tuna eye
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